FIG. 2.

Effect of heat and trypsin treatment on the Western immunoblot reactivity of anti-OmpA MAbs. Triton-insoluble cell envelope of serovar Typhimurium strain SH5014 before (lanes 1 to 4) and after (lanes 5 and 6) treatment with trypsin was suspended in SDS sample buffer and then either boiled at 100°C for 5 min (lanes 1, 3, 5, and 6) or solubilized at 37°C for 1 h (lanes 2 and 4). Proteins were separated by SDS-PAGE and immunoblotted (Fig. 1). Nitrocellulose strips were probed with either MAb SH16.2 (lanes 1, 2, and 5) or Ag.8 ascites (lanes 3, 4, and 6).