FIG. 4.

CNBr cleavage of OmpA and reaction of the fragments with MAb SH16.2. The CNBr-generated peptides from Salmonella and E. coli were separated by SDS-PAGE and immunoblotted (Fig. 1). Lanes 1 and 4 contain the CNBr peptides as separated on the SDS gel, lanes 2 and 5 contain the immunoblots of MAb SH16.2 with these peptides, and lanes 3 and 6 contain the control strips probed with ascites fluid from Ag.8.