TABLE 1.
Reactivities of anti-OmpA MAbs with Salmonella OmpA, OM, and whole cells in ELISA
| MAba | Isotype | MAb reactivityb
|
|||
|---|---|---|---|---|---|
| OmpAc | OMc | SH5014 WC | SA1627 WC | ||
| PA 24.1 | IgG2b | +++ | +++ | ++ | ++ |
| SH 16.2 | IgG2a | +++ | +++ | +++ | ++ |
| SH 68.8 | IgG2a | +++ | +++ | +++ | +++ |
| SH 267.15 | IgG2a | +++ | +++ | +++ | +++ |
| SH 269.1 | IgG2b | +++ | +++ | +++ | +++ |
| SH 333.12 | IgG2a | +++ | +++ | +++ | ++ |
| SH 377.16 | IgG2b | +++ | +++ | +++ | + |
| SH 471.7 | IgG2b | +++ | +++ | +++ | +++ |
| SH 591.1 | IgG2a | +++ | +++ | +++ | +++ |
| SH 621.1 | IgG2b | +++ | +++ | +++ | +++ |
| SH 720.1 | IgG2a | +++ | +++ | +++ | +++ |
| SH 785.1 | IgG2b | +++ | +++ | +++ | +++ |
MAbs with the prefixes PA and SH were produced from mice immunized with purified OmpA and heat-killed SH 5014 whole cells, respectively.
Antibodies for ELISA were used at a dilution which resulted in an optical density at 405 nm within the range of 1.0 to 1.5 when tested with homologous antigen (immunogen) as previously described (37). Absorbance values for ascites from the cell fusion partner P3 × 63-Ag8.653, which is a nonsecretor of Igs (control), were always <0.10 at the antibody dilutions tested. Similarly, the absorbance values for the anti-OmpA MAbs against Salmonella porins, LPS, and whole cells of strain SL 1917 (OmpA−) were also <0.1 at the antibody dilutions tested. Abbreviations: OM, outer membrane; WC, whole cells. Symbols for ELISA reactions: +, weakly positive (absorbance, 0.25 to 0.5); ++, moderately positive (absorbance, 0.5 to 1.0); +++, strongly positive (absorbance, >1.0).
OmpA and OM were isolated and purified from S. enterica serovar Typhimurium strain SH5014.