FIG. 3.

Growth of Y. pestis KIM6 (Ybt⁻ Yfe⁺ TonB⁺ HasB⁺), KIM6-2031.1 (Ybt⁻ Yfe⁻ TonB⁺ HasB⁺), KIM6-2073 (Ybt⁻ Yfe⁺ TonB⁻ HasB⁺), and KIM6-2073.1 (Ybt⁻ Yfe⁻ TonB⁻ HasB⁺) at 30°C across PMH plates containing a gradient of the iron chelator conalbumin (0 to 5 μM) (1). Bars represent incremental growth against the concentration gradient over a 72-h period. Growth distances were recorded in millimeters from 0 (no growth) to 90 (confluent growth). The data shown are from a single growth assay but are representative of three independent experiments.