TABLE 1.
Analysis of hemin or hemoprotein utilization by various Y. pestis strainsa
| Heme source | Growth response of:
|
||
|---|---|---|---|
| KIM6+ (Hmu+ TonB+) | KIM6-2060.1+ (Hmu− TonB+) | KIM6-2073+ (Hmu+ TonB−) | |
| 500 μM hemin | 3.1 | NG | NG |
| 50 μM hemin | 1.9 | NG | NG |
| 50 μM hemin-200 μM albumin | 1.6 | NG | NG |
| 200 μM albumin | NG | NT | NT |
| 50 μM hemoglobin | 3.7 | NG | NG |
| 10 μM hemoglobin | 2.9 | NG | NG |
| 50 μM hemoglobin-100 μM haptoglobin | 2.7 | NG | NG |
| 500 μM myoglobin | 3.9 | NG | NG |
| 100 μM hemopexin | 2.9 | NG | NG |
| 100 μM apohemopexin | NG | NG | NG |
a
Approximately 107 cells were overlaid onto deferrated PMH-100 μM EDDA agarose plates. Twenty microliters of the indicated compounds were added to 0.3-cm wells cut into the agarose plates, and growth around the wells (diameters in centimeters) was recorded after 3 days of incubation at 37°C. The results are averages from two independent experiments; NG, no growth in both independent experiments; NT, not tested.