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. 2000 Mar 14;97(7):3089–3093. doi: 10.1073/pnas.040559597

Figure 3.

Figure 3

Simultaneous flavin attachment and appearance of catalytic activity on reconstitution of mutant enzyme with 8-methylsulfonyl FAD. The flavin attachment was monitored by the increase in absorbance at 460 nm (○) under the conditions described in the legend of Fig. 2. During this process, 5-μl aliquots were withdrawn and immediately assayed for DAAO activity (█).