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RT-PCR analysis of GBS strains A909 and AJ200. cDNA was PCR amplified with primers specific for rpoE (A), ropA (B), and pyrG (C). The position of the expected product is indicated with an arrow. Lanes 1 and 2, A909; lanes 3 and 4, AJ200; lane 5, no-template control; lane 6, DNA control; lane 7, DNA standards. Reverse transcriptase was omitted from samples in lanes 1 and 3, which serve as a control for DNA contamination in the RNA samples.
