TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| S. agalactiae | ||
| A909 | Wild-type, serotype Ia | 21 |
| AJ8D3 | A909 Tn917::rpoE | 13 |
| AJ200 | A909ΔrpoE, allelic exchange mutant, Kmr | This study |
| A909ΔcpsE | A909ΔcpsE, acapsular mutant | L. Madoff |
| E. coli MC1061 | F− araD139 Δ(ara-leu)7696 Δ(lac)X74 galU galK hsdR2 (rK− mK+) mcrA mcrB1 rpsL(Strr) | 35 |
| Plasmids | ||
| pDC123 | Cmr, phoZ, cloning vector | 5 |
| pCIV2 | Kmr/ΩKm-2 | 25 |
| pHY304 | Emr temperature-sensitive pVE6007Δ derivative, lacZα/multiple cloning site of pBluescript | H. H. Yim |
| pAJ020 | pDC123 with 2.5-kb insert containing rpoE gene and flanking DNA sequence | This study |
| pAJ024 | Kmr pAJ020 with rpoE deleted and replaced with ΩKm-2 from pCIV2 | This study |
| pAJ042 | Emr Kmr pHY304 with insert containing ΩKm-2 and flanking DNA sequence | This study |