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. 2003 Jul;71(7):3714–3723. doi: 10.1128/IAI.71.7.3714-3723.2003

FIG. 3.

FIG. 3.

C. burnetii icmW homologous gene complements an L. pneumophila icmW mutant for intracellular growth. Intracellular growth experiments in the protozoan host A. castellanii were performed as described in Materials and Methods. (A) Wild-type L. pneumophila JR-32 (⧫) and the icmW mutant (GY141) containing the vector pMMB207αb-Km-14 (▪), the L. pneumophila icmW gene (pZT-W-01) (▵), the L. pneumophila icmWX operon (pZT-WX-01) (▴), and the C. burnetii icmWX homologous genes under regulation of the L. pneumophila icmWX regulatory region (pZT-lpncox-WX) (•) were used. (B) icmX mutant LELA3993 containing the same plasmids (without pZT-W-01) was used. The experiments were performed three times, and similar results were obtained each time.