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DNA substrates used to assay Pol η fidelity. The nondamaged and damaged DNA substrates are shown. The position of the cis-syn T–T dimer in substrates S-3 and S-4 is indicated by a ∨. The first T and second T of the cis-syn T–T dimer or the identical nondamaged sequence refer to the first and second T residues encountered by the DNA polymerase by using that strand as a template.
