Table 1.

Fidelity of Polη on nondamaged and damaged DNA templates

dNTP	Vmax, nM/min	Km, μM	Vmax/Km	finc
Incorporation opposite the first nondamaged T (substrate S-1; Fig. 1)
dGTP	0.14  ± 0.0095	30  ± 0.80	0.0047	8.1  × 10−3
dATP	1.0  ± 0.082	1.7  ± 0.50	0.58	NA
dTTP	0.17  ± 0.018	47  ± 11	0.0036	6.2  × 10−3
dCTP	0.11  ± 0.0043	31  ± 4.7	0.0035	6.0  × 10−3
Incorporation opposite the second nondamaged T (substrate S-2; Fig. 1)
dGTP	0.070  ± 0.0036	61  ± 9.8	0.0011	1.4  × 10−3
dATP	0.68  ± 0.042	0.89  ± 0.22	0.76	NA
dTTP	0.087  ± 0.0036	31  ± 4.8	0.0028	3.7  × 10−3
dCTP	0.10  ± 0.0059	20  ± 4.9	0.0050	6.6  × 10−3
Incorporation opposite the first damaged T (substrate S-3; Fig. 1)
dGTP	0.19  ± 0.0095	56  ± 8.8	0.0034	5.2  × 10−3
dATP	0.40  ± 0.082	0.62  ± 0.14	0.65	NA
dTTP	0.14  ± 0.019	220  ± 67	0.00064	1.0  × 10−3
dCTP	0.14  ± 0.0081	26  ± 5.9	0.0054	8.3  × 10−3
Incorporation opposite the second damaged T (substrate S-4; Fig. 1)
dGTP	0.34  ± 0.0091	114  ± 12	0.0030	4.6  × 10−3
dATP	0.47  ± 0.019	0.73  ± 0.11	0.65	NA
dTTP	0.52  ± 0.056	1300  ± 290	0.00040	6.2  × 10−4
dCTP	0.47  ± 0.013	170  ± 17	0.0028	4.3  × 10−3

NA, not applicable.