TABLE 1.
Effect of TLR2 deficiency in the C3H/HeJ mouse humoral immune response to B. burgdorferi infectiona
| Genotype | OD490 for Ig (dilution)b:
|
VlsE-specific IgG (1:200) | ||||
|---|---|---|---|---|---|---|
|
B. burgdorferi isotype
| ||||||
| IgG (1:1,000) | IgG1 (1:50) | IgG2a (1:50) | IgG2b (1:500) | IgG3 (1:50) | ||
| C3H/HeJ | 0.146 ± 0.041 | 0.070 ± 0.007 | 0.067 ± 0.017 | 0.131 ± 0.011 | 0.066 ± 0.040 | 3.21 ± 0.154 |
| TLR2−/− C3H | 0.284 ± 0.122* | 0.138 ± 0.042* | 0.108 ± 0.034* | 0.091 ± 0.02* | 0.067 ± 0.024 | 3.38 ± 0.161 |
| Uninfected | 0.003 ± 0.005 | 0.006 ± 0.006 | 0.002 ± 0.003 | 0 | 0.001 ± 0.001 | 0.094 ± 0.035 |
a
Sera were collected from seven C3H/HeJ and seven TLR2−/− C3H/HeJ mice 4 weeks post-B. burgdorferi infection.
b
Values indicate the mean ± standard deviation OD490 for each group of sera. Sera were diluted as shown in parentheses. The diluted sera were incubated on plates coated with either B. burgdorferi or C6 peptide and developed with Ig isotype-specific HRP-conjugated antibodies. *, difference between TLR2+/+ and TLR2−/− significant by Student's t test (P < 0.01).