TABLE 1.
Site-specific PCR primers of the OmpB451-846 fragment
| Peptide | Forward Primer | Reverse primer |
|---|---|---|
| OmpB458-652 | 5′-ATGATTACAGCAATTGAAGCATCAGGTG-3′ | 5′-CGTCATTTCCAATAACTAACTCGT-3′ |
| OmpB595-744 | 5′-ATGACAGGCGTTGTTGATGCGAGTAG-3′ | 5′-CGGCATTAAAGACGAAAGAACCT-3′ |
| OmpB739-848 | 5′-ATGTTCGTCTTTAATGCCGGTGGAA-3′ | 5′-CAATCACTACGTTACCGGGACCAGAA-3′ |
| OmpB595-654 | 5′-ATGGGCGTTGTTGATGGGAGTAG-3′ | 5′-CTGCACCGTCATTTCCAATAACTA-3′ |
| OmpB645-692 | 5′-ATGGAGTTAGTTATTGGAAATGACG-3′ | 5′-TTGTACCGGCTGCAAGAGTTGTA-3′ |
| OmpB689-744 | 5′-ATGGCCGGTACAAATTTAGGTAGTG-3′ | 5′-CGGCATTAAAGACGAAAGAAC-3′ |