Figure 5.

Activation of the Shh signaling pathway drives Vax2 nuclear accumulation and is dependent on Ser 170. Wild-type or S170A/D mutant forms of Myc-tagged Vax2 was expressed in Schwann cells—with or without constitutively active Flag-Smo-M2 (Incardona et al. 2002) and with and without the Shh inhibitor cyclopamine. Cells were treated with 200 nM cyclopamine for 4 h prior to fixation. Cells were costained with rabbit anti-Vax2 antibody (green) and goat anti-pCREB antibody (red), or rabbit anti-Vax2 antibody (green) and mouse anti-Flag antibody (red). Nuclei were visualized with DAPI (blue).