Skip to main content
. 2006 Oct 15;20(20):2820–2832. doi: 10.1101/gad.1461206

FIGURE 6.

FIGURE 6.

Rictor knockout inhibits Akt phosphorylation. (A) Akt phosphorylation but not S6K1 phosphorylation is inhibited in Rictor knockout. Rictor knockout embryos from heterozygous intercrosses at E8.5E9.5 were dissected. At the age of either E8.5 or E9.5, one wild-type (+/+), one homozygous knockout (−/−), and two heterozygous (+/−) embryos were analyzed by Western blotting for phosphorylation and protein levels as indicated. (B) Decreased hSin1 protein levels in Rictor knockout embryos. Samples were prepared as described in A. Each group of wild-type and knockout samples was prepared at the same time and analyzed by Western blotting in the same panel. Different sample groups were collected at different times. (C) Decreased Akt phosphorylation in the liver tissues of Rictor+/− heterozygous knockout mice. The mice liver tissues were collected at 2 wk of age. Samples from two wild-type (+/+) and two heterozygous (+/−) mice were analyzed by both Western blotting (top two panels) and PCR genotyping (bottom two panels).