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. 2003 Jul;71(7):3794–3801. doi: 10.1128/IAI.71.7.3794-3801.2003

TABLE 1.

Primers used to test for the expression of mas, msl, and pps genes by RT-PCRa

ORF(s) Primer sequences (5′→3′)
ppsA and -B CGCAGTGCCCGACGACATCA, GTCGAGTGAGTTCCGGGCAC
ppsB and -C GCCGAGAGGTCGGTGTGATGC, AGCATTCGCTGCTGCGGGTCC
ppsC and -D AATGACCGCAGCGACACCAGATCG, TAGCCGGCCCGCCAGCGTGAGCAT
ppsD and -E ATGACAAGTCTGGCGGAGCGCGCG, TCCGGTGCTCAGGTACGGGTCGAT
mas GCCGGATCTGCTCGGCGAAC, ATCGTGTATGGGCCTCGCGT
msl1 TACCGTCGCCGTACACCCAT, TGAACGGTGCGGCCACCAAA
msl2 GCCGAGCACCCTCACCTGCG, GAAGATCTCAGCGCCCGCCG
msl3 TGGAGATCCGGTCGAATACGC, GTCAAAACTGCTCGCCGTCAC
msl4 TACCGAAGCATCCGCCAGCCG, ACAACCCACCTCGTCACCGGC
msl5 GCGCGGGCGCCCTTGAGGAT, GTCCGGCCTCCATCAAGTCG
msl6 CGCACTGCGAGCCCATGCGGT, AAGCCTTCTACCGGCTCGGCG
msl7 GTATGCGGCCACCCACAC, CGCGCAGCGTGTCCCCAC
a

For pps, the primers used amplified the junctions between two consecutive ORFs.