TABLE 1.
ORF(s) | Primer sequences (5′→3′) |
---|---|
ppsA and -B | CGCAGTGCCCGACGACATCA, GTCGAGTGAGTTCCGGGCAC |
ppsB and -C | GCCGAGAGGTCGGTGTGATGC, AGCATTCGCTGCTGCGGGTCC |
ppsC and -D | AATGACCGCAGCGACACCAGATCG, TAGCCGGCCCGCCAGCGTGAGCAT |
ppsD and -E | ATGACAAGTCTGGCGGAGCGCGCG, TCCGGTGCTCAGGTACGGGTCGAT |
mas | GCCGGATCTGCTCGGCGAAC, ATCGTGTATGGGCCTCGCGT |
msl1 | TACCGTCGCCGTACACCCAT, TGAACGGTGCGGCCACCAAA |
msl2 | GCCGAGCACCCTCACCTGCG, GAAGATCTCAGCGCCCGCCG |
msl3 | TGGAGATCCGGTCGAATACGC, GTCAAAACTGCTCGCCGTCAC |
msl4 | TACCGAAGCATCCGCCAGCCG, ACAACCCACCTCGTCACCGGC |
msl5 | GCGCGGGCGCCCTTGAGGAT, GTCCGGCCTCCATCAAGTCG |
msl6 | CGCACTGCGAGCCCATGCGGT, AAGCCTTCTACCGGCTCGGCG |
msl7 | GTATGCGGCCACCCACAC, CGCGCAGCGTGTCCCCAC |
For pps, the primers used amplified the junctions between two consecutive ORFs.