TABLE 2.
Lipids | % 14C in:
|
|
---|---|---|
Wild type | msl6 mutant | |
DIM | 30 | 0 |
Fatty acids | 28 | 0 |
Hydroxy fatty acids | 2 | 0 |
PAT-I | 16 | 17 |
Fatty acids | 14 | 13 |
Mycolipanolic acids | 1.7 | 3 |
Hydroxyphthioceranic acids | 0.3 | 1 |
PAT-II | 4 | 3 |
Fatty acids | 1 | 0.8 |
Mycolipanolic acids | 1 | 0.6 |
Hydroxyphthioceranic acids | 2 | 1.6 |
PAT-III | ND | 7 |
Fatty acids | ND | 1 |
Mycolipanolic acids | ND | 0.6 |
Hydroxyphthioceranic acids | ND | 5.4 |
SL | 45 | 68 |
Fatty acids | 10 | 10 |
Mycolipanolic acids | 4 | 6 |
Hydroxyphthioceranic acids | 31 | 52 |
Very polar eipids | 5 | 4 |
Fatty acids | 0.5 | 0.5 |
Mycolipanolic acids | 0.3 | 0.2 |
Hydroxyphthioceranic acids | 4.2 | 3.3 |
PAT, polyacyl trehalose; SL, sulfolipids; very polar lipids, lipids that remained in the origin and diacyl trehaloses that remained near the origin in TLC using 10% methanol in chloroform as the solvent system. ND, not detected.