Figure 3.

Conditional loss of RBP-J does not disrupt basal gene expression but does decrease proliferation. (A–D) Basal markers Keratin 5, Keratin 14, p63, and integrins are not altered in RBP-J KO epidermis. Immunostaining (A,B), FACS analysis (C), and RT–PCR (D) for basal markers shows that these markers are maintained if not slightly increased in the absence of RBP-J KO. (E,F) Staining and quantification of BrdU incorporation (E) and phospho-Histone H3 (F) demonstrates diminished proliferation in RBP-J KO epidermis. (G) Comparable immunofluorescence between wild-type (WT) and RBP-J cKO newborn skin stained with Abs against Keratin 6 (K6), a marker of hyperproliferative disorders in the epidermis.