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. 2006 Nov 1;20(21):3010–3021. doi: 10.1101/gad.1493506

Figure 2.

Figure 2.

Enforced GATA-2 instructs GMPs to exclusively select the eosinophil fate. (A) Clonogenic analyses of GMPs with or without GATA-2 transduction. Purified GMPs were transduced with a GFP-tagged retrovirus carrying a murine GATA-2 cDNA or an empty control retro-virus. GFP-positive GMPs were isolated 36 h after the retroviral infection and subjected to methylcellulose assays. Types of colonies were evaluated at day 5 by May-Giemsa staining. Cytokines added are indicated. (B) Progeny of GMPs transduced with the GATA-2 retrovirus. An image of fluorescence microscopy (GFP filter, ×1000, upper panel), and that of May-Giemsa staining (×1000, bottom panel) are shown. Cells positive for GFP possessed eosinophilic granules. (C) The phenotypic analysis of progeny of GMPs transduced with GATA-2. Although BMCPs gave rise to FcεRIα+ basophils and mast cells in the culture, GATA-2+ GMPs did not give rise to FcεRIα+ cells. (D) Gene expression analyses of GMPs immediately after the completion of GATA-2 transduction. (E) Real-time PCR analysis of GATA-2 mRNA before and after GATA-2 transduction. ΔCt values for each samples were standardized by GAPDH Ct values. GATA-2 transduction experiment was performed three times using GMPs purified independently, and the same results were obtained.