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. 2006 Nov 1;20(21):3010–3021. doi: 10.1101/gad.1493506

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Figure 6. — Expression profiles of transcription factors and lineage-related genes in CLPs after two-step retroviral transduction of C/EBPα and GATA-2. (A)RT–PCR analyses of lineage-related gene expression in CLPs purified immediately after the completion of two-step retroviral transduction. R1–R4 represent sorting regions defined in Figure 5. (B) Change in the expression level of C/EBPα and GATA-2 mRNA in CLPs after tranduction of control-GFP, C/EBPα, or GATA-2, determined by a real-time PCR assay. ΔCt values for each samples were standardized by GAPDH Ct values. (C) Change in the expression level of lymphoid-affiliated EBF, Pax-5, and GATA-3 in CLPs after tranduction of control-GFP, C/EBPα, or GATA-2. Representative data of three independent experiments are shown.