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. 2006 Nov 1;20(21):2996–3009. doi: 10.1101/gad.1483906

Figure 5.

Figure 5.

Effect of depletion of LRP130 on endogenous gene expression. Primary hepatocytes were depleted of endogenous LRP130 using an RNAi construct designated siLRP130 or a scrambled control, siControl. Transcription of genes responsive to PGC-1α coactivation and implicated in gluconeogenesis, heme metabolism, mitochondrial respiration, and fatty acid oxidation was measured using RT quantitative PCR. (A) Forced expression of siControl or siLRP130. (B) Coforced expression of either siControl/GFP (siControl), siControl + PGC-1α, or siLRP130 + PGC-1α. (C) Forced expression of siControl or siLRP130 in the presence or absence of dexamethasone and forskolin (Dex/Fsk). Experiments were performed in triplicate and are respresentative of n = 3 independent biological experiments. (*) p < 0.05; (**) p < 0.01; (***) p < 0.001. Asterisks designate statistical comparisons made between siControl and siLRP130 (A) siControl + PGC-1α and siLRP130 + PGC-1α (B), and siControl + Dex/Fsk and siLRP130 + Dex/Fsk (C). (#) p < 0.01; stastistical comparisons are made between siControl and siControl + PGC-1α (B), and siControl and siControl + Dex/Fsk (C). The control for Dex/Fsk contained an appropriate vehicle. Error bars represent standard deviation.