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. 2006 Nov 1;20(21):2943–2948. doi: 10.1101/gad.1461706

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Figure 3. — MTA3 status affects Wnt4 expression and signaling. (A) Expression of T7-MTA3 protein in the indicated pooled HC11 clones. (B) Northern blot analysis of the Wnt4 mRNA. (C) Western blot showing Wnt4 protein in the culture media. (D) Western blot analysis of β-catenin and Ser9 phosho-GSK3β. (E) Top-flash-Luc activity in the HC11 clones. (RLU) Relative light unit. (F) RT–PCR analysis of the WISP2 mRNA. (G,H) Effect of MTA3-siRNA on the levels of Wnt4 and WISP2 mRNAs. (I) Effect of TSA on the levels of Wnt4 mRNA by RT–PCR. (J) Effect of TSA on secreted Wnt4 protein.