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. 2005 May;166(5):1515–1522. doi: 10.1016/s0002-9440(10)62367-x

Figure 5.

Figure 5

PAF stimulates phosphorylation of β-catenin in KS cells. Subconfluent cultures of KS cells were starved for 12 hours and then incubated with PAF (10 and 50 ng/ml) at the indicated times. Cells were also treated with the vehicle alone (C), or with PAF (50 ng/ml) plus PAF-receptor antagonists Ginkgolide B (G; 0.34 μmol/L) or CV 3988 (CV; 3 μmol/L) for 1 hour. A: Cells extracts were subject to immunoprecipitation with antibody against β-catenin. Precipitated proteins were analyzed by SDS-PAGE followed by immunoblotting with an antibody to phosphotyrosine. PY-β-catenin, tyrosine phosphorylated β-catenin. B: Western blotting analysis using the anti-phospho-S33/S37/T41 antibody to β-catenin. The data shown are representative of three different experiments.