FIG. 2.

Distribution of Rgp protease activity in P. gingivalis. Activities against BAPNA were tested in whole-cell culture (WC), cell-free medium (CF), cell suspension (CS), vesicles (V), and vesicle-free medium (VF). The fractions were prepared according to the methods of Potempa et al. (36). One hundred percent of the activities in the whole-cell fraction were assumed to be produced by that strain. The activities measured in P. gingivalis FLL92 represent 10 and 60% of the activities measured in the wild-type strain W83 in exponential (OD₆₆₀ = 0.8) and stationary (OD₆₆₀ = 1.5) growth phases, respectively. CF + CS = WC; VF + V = CF.