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. 2003 Jul;71(7):3740–3747. doi: 10.1128/IAI.71.7.3740-3747.2003

FIG. 8.

FIG. 8.

Detection of proteolytic activity in casein-conjugated polyacrylamide gel. All lanes contained 20 μg of acetone (37.5%)-precipitated proteins from the supernatant fractions of cultures in BHI medium at exponential growth phases. Samples were solubilized at room temperature for 10 min in a Tris-glycine SDS sample buffer (Invitrogen) prior to electrophoresis. Lanes: 1, W83; 2, FLL92. The arrow indicates a 48-kDa proteolytic band present in the FLL92 exponential-growth-phase extracellular proteins.