FIG. 1.
SspH1 is localized to the nucleus in mammalian cells. Intestine-407 cells were infected with S. enterica serovar Typhimurium expressing SspH1-HA-HA (A) or SseJ-HA-HA (B). Cells were fixed, permeabilized, and immunostained for HA (red) and Salmonella LPS (green). Cell nuclei were visualized by staining with DAPI (blue). Raw-TT10 (C) and CHO-K1 (D) cells were transiently transfected with constructs expressing SspH1-HA, SlrP-HA, or SspH2-HA. HA-tagged proteins were detected by immunofluorescent staining (red), while DNA was stained with DAPI (blue).