FIG. 3.

The Shigella LPX repeat protein IpaH9.8 also inhibits NF-κB-dependent reporter gene expression. CHO-K1 cells were transiently transfected with pELAM-1-fluc, pRL-TK, pCDNA3.1/Zeo/mCD14, pNeo/Tak, and an empty vector or a construct expressing one of the following LPX repeat proteins: SspH1 (A and B), SlrP (A), SspH2 (A), or IpaH9.8 (B). After 24 h, the cells were incubated with E. coli LPS (solid bars) or fresh medium (open bars) for 5 h, and luciferase activity was measured. Firefly luciferase activity was normalized to the activity of Renilla luciferase. Data are represented as the mean ± standard deviation of triplicate samples. Each LPX repeat protein-expressing vector is diagramed to the right of its corresponding empty vector.