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. 2000 Mar 14;97(7):3112–3117. doi: 10.1073/pnas.040560597

Figure 3.

Figure 3

Expression of CepA constructs in E. coli: (A) CepA1-575-His6; (B) His6-CepA1-1596-His6; (C) MBP-CepA1-575-His6; (D) CepA1-1596-CBD-His8; and (E) CepA1-3158-His6. For all gels: lanes 1 and 2, whole-cell lysates from uninduced and isopropyl β-d-thiogalactoside-induced cultures, respectively, of E. coli strain BL21(DE3)pLysS; lane 3, soluble fraction from the induced cells; and lane 4, protein purifed by Ni2+-affinity chromatography (or amylose affinity chromatography for MBP-CepA1-575-His6). M, molecular mass markers (200, 116, 97, 66, and 45 kDa). Protein samples were resolved by 10% Tris⋅glycine SDS/PAGE except for E Left, for which a 5% gel was used.