TABLE 2.
Effect of rDiAgs on IgG2c production by MLN cells in vitroa
| Mean IgG2c production (ng/ml) ± SD in:
|
|||||||
|---|---|---|---|---|---|---|---|
| Medium | V1 | V2 | V1V2 | V2V1 | V1V1 | V2V2 | |
| Control MAb | 4.1 ± 0.8 | 3.5 ± 0.2 | 3.3 ± 0.4 | 3.7 ± 0.4 | 3.6 ± 0.6 | 3.8 ± 0.5 | 3.5 ± 0.7 |
| αIL-10 MAb | 4.3 ± 0.5 | 3.8 ± 0.4 | 3.9 ± 0.5 | 4.1 ± 0.6 | 3.9 ± 0.5 | 4.1 ± 0.5 | 3.9 ± 0.4 |
a
Naive MLN cells (2 × 106/ml) were cultured with each rDiAg (3.3 μg/ml) in the presence of anti-IL-10 MAb or isotype-matched control MAb (5 μg/ml) for 8 days. IgG2c levels in culture supernatants were measured by ELISA. The mean and standard deviations are from five experiments.