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. 2003 Jul;71(7):3875–3884. doi: 10.1128/IAI.71.7.3875-3884.2003

FIG. 5.

FIG. 5.

Cross-reactive binding and opsonic killing activity of rabbit and mouse antisera raised to MEP-KLH conjugate vaccine. (A) IgG titers against the vaccine strain (strain 2192) and multiple mucoid, LPS rough P. aeruginosa isolates from CF patients. (B) Opsonic activity of a serum dilution of 1:10 of the rabbit and mouse sera after immunization with MEP-KLH conjugate against the same target strains. No significant difference was found in binding to MEP isolated from the vaccine strain or heterologous strains of mouse and rabbit antisera. Also, the opsonic activity of the antisera against heterologous mucoid strains of P. aeruginosa did not differ significantly from their activity against strain 2192, except for strain 258, for which the killing activity was significantly lower than that against the vaccine strain (P < 0.001 [as determined by ANOVA with the Dunnet test for comparison with strain 2192]). The sera tested were obtained from a hyperimmunized rabbit and from five C3H-HeN mice 35 days after the first immunization. The mouse sera were pooled for analysis. MEP from strain 258 was not tested by ELISA for cross-reactive IgG, since this strain reverts to the nonmucoid phenotype when grown in the Mian’s medium used to produce MEP. The bars represent the mean of titers of two replicates determined by linear regression (A) and the means of four replicates (B); error bars indicate the standard error of the mean in panel B.