FIG. 7.

Effect of acetate substituents on the binding of conjugate-induced antisera to MEP. (A) Binding of rabbit IgG after immunization with the MEP-KLH conjugate vaccine to MEP and de-O-acetylated MEP as the coating antigen in an ELISA. (B) Competitive inhibition ELISA with native MEP and de-O-acetylated MEP to inhibit binding of conjugate-induced antibody to native MEP as the solid-phase antigen. The percent inhibition was determined by comparison of the absorbance at 405 nm in the presence or absence of inhibitor. Native and de-O-acetylated MEP used for coating and inhibition were derived from P. aeruginosa strain 2192.