FIG. 1.

Identification of Tn4001 insertions within the genomes of M. gallisepticum HA⁻ transformants. Lanes: 1, wild-type M. gallisepticum R-low; 2 to 4, M. gallisepticum mutants E117, E345, and E325, respectively. In each lane, 2 to 4 μg of chromosomal DNA was digested with EcoRI (A) or XbaI (B and C) restriction enzymes, subjected to Southern blot hybridization, and probed with the ³²P-labeled gentamicin gene (A), gp-1 fragment (B), or cm-1 fragment (C). The sizes of the hybridization bands are shown on the left.