FIG. 2.

Identification of Tn4001 insertion sites within the M. gallisepticum R-low gapA-crmA operon. The solid line represents an 8-kb genomic fragment of M. gallisepticum R-low that was cloned and sequenced. The locations and directions of the mgc-2, gapA, and crmA genes are indicated by large labeled arrows. The positions of HaeIII (Ha), HindIII (H), XbaI (X), and EcoRI (E) restriction sites are indicated. The positions of Tn4001 insertions in mutants E117, E345, and E325 are indicated by labeled arrows. The locations of the gp-1 and cm-1 genomic fragments used as probes are indicated by broken lines. Two XbaI genomic fragments (shown in Fig. 1) are indicated by dotted brackets along with their corresponding sizes.