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Western blot analysis of M. gallisepticum R-low Tn4001 mutants. Total cell proteins from wild-type M. gallisepticum R-low (lane 1), mutants E117, E345, and 325 (lanes 2 to 4, respectively), and M. gallisepticum R-high (lane 5) were subjected to SDS-PAGE and immunoblotted with monospecific anti-GapA (A) or anti-CrmA (B) antibodies. The 105-kDa GapA and 116-kDa CrmA protein bands are indicated.
