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. 2003 Jul;71(7):3812–3820. doi: 10.1128/IAI.71.7.3812-3820.2003

FIG. 6.

FIG. 6.

Monitoring the presence and genomic location of the Tn4001 transposon in M. gallisepticum R-low mutants. (A) Tn-position-PCR of various mutants. Primers ISb1 and GAf4 were used to amplify the junction between the IS256 arm of the Tn4001 transposon and the gapA gene in M. gallisepticum R-low mutants E117, E345, and E325 (lanes 1 to 3, respectively). The sizes of the PCR products corresponding to the mutants are shown on the left. (B) Tn-position-PCR of mutant E117 isolated directly from the trachea at 3, 7, 11, 21, and 28 days p.i. (lanes 1 to 5, respectively) with primers ISb1 and GAf4. The 0.25-kb PCR product is indicated. (C). Western blot analysis of total cell proteins from mutant E117 (depicted in panel B) with anti-CrmA antibodies. The 116-kDa CrmA protein band is indicated. The HA phenotype of colonies from each isolate on agar plates (positive or negative, + or −, respectively) is shown at the bottom.