TABLE 1.

Primers used for PCR and to amplify Tn4001 insertion sites

Primer designation	Sequence	Gene	Orientation	Reference(s) or source
ISb1	5′-AAGTCCTCCTGGGTATGT-3′	IS256 arm of Tn4001	Reverse	5, 18
GAf4	5′-GGTGGTAACGGTACATGAGTT-3′	gapAa	Forward	This studyb
gapA3F	5′-TTCTAGCGCTTTARCCCTAAACCC-3′	gapAa	Forward	This studyb
gapA4R	5′-CTTGTGGAACAGCAACGTATTCGC-3′	gapAa	Reverse	This studyb
GENTA5	5′-TATGAAAAAGGTGATAAATAAATG-3′	Gentamicin	Forward	6
GENTA6	5′-AATTTCTGGTGTTAAAAAAGTTCC-3′	Gentamicin	Reverse	6
MG13	5′-GCTTCCTTGCGGTTAGCAAC-3′	16S rRNA of M. gallisepticum	Forward	22
MG14	5′-GAGCTAATCTGTAAAGTTGGTC-3′	16S rRNA of M. gallisepticum	Reverse	22
MSL1	5′-GAGAAGCAAAATAGTGATATCA-3′	16S rRNA of M. synoviae	Forward	22
MSL2	5′-CAGTCGTCTCCGAAGTAAACAA-3′	16S rRNA of M. synoviae	Reverse	22

^aGenBank accession number AF214004.

^bM. Garcia, University of Georgia, personal communication.