Figure 2.

Tandem reiteration of the VN8 module generates transcriptional activators of widely differing potencies. (A) Structure of synthetic activators used in this study. Each activator carried the GAL4 DBD fused to 1×, 2×, 3×, or 6× copies of the wild-type VN8 sequence, or three copies of mutant VN8 sequence (3×M). (B) Relative transcriptional potency of each activator. GAL4-fusion proteins were assayed for transcriptional activation in human HeLa cells as described in Materials and Methods. Relative transcriptional activity is shown on a logarithmic scale, setting the transcriptional activity of the GAL4 DBD alone (Δ) at 1.