FIG. 4.

Detection of anti-rA2- or anti-rLACK-specific antibodies in sera of immunized BALB/c mice after challenge infection. rA2 (lanes 1 and 3) and rLACK (lanes 2 and 4) were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred onto nitrocellulose membranes, and incubated with sera of mice immunized with rA2-rIL-12 (lane 1), rLACK-rIL-12 (lane 2), or rLACK-rA2-rIL-12 (lanes 3 and 4). The anti-A2 monoclonal antibody C9 and a rabbit anti-rLACK polyclonal serum were used as positive controls (data not shown).