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Levels of parasite-specific IgG isotypes in sera of immunized BALB/c mice prior to (A) and 2 months after (B) challenge infection with L. amazonensis. IgG1 and IgG2a levels were assessed by ELISA in sera, diluted 1:100, from control and immunized mice as described in Materials and Methods. Bars represent the averages, with error bars representing standard deviations, of levels determined for eight mice per group. Abs, absorbance.
