FIG. 6.

Association of the avg-B₂ promoter with avg genes in a subpopulation of cells present in each subclone: PCR amplification of M. agalactiae isolates. Total genomic DNAs of the M. agalactiae PG2 type strain (A), clonal isolate 627#3 (B), clonal isolate 627#4 (C), and clonal isolate 627#5 (D) were subjected to PCR amplification across the junction between the avg-B₂ promoter and five avg structural genes. The following primer pairs were used: for the avgA gene, pp-1 and pa-2 (lane 1); for the avgB gene, pp-1 and pb-1(lane 2); for the avgC gene, pp-1 and pc-2 (lane 3); for the avgD gene, pp-1 and pd-1 (lane 4); and for avgE gene, pp-1 and pe-1 (lane 5). The size of each PCR product (in base pairs) is indicated above the band.