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Induction of ClpL in clpP mutant. Exponentially growing S. pneumoniae CP1200 (A550 = 0.3) and its isogenic clpL and clpP derivatives were heat shocked at 42°C for 30 min. Proteins from 3 ml of culture were subjected to immunoblot analysis with either anti-ClpL or anti-ClpP polyclonal serum. The positions of ClpL and ClpP are indicated.
