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(A) SDS-PAGE of in vitro-reassembled holotoxin after purification on immobilized d-galactose. Lanes: 1, flowthrough; 2, eluate 1; 3, eluate 2; 4, eluate 3. (B) SDS-PAGE and Western blot with anti-CTA and anti-CTB antibodies. Lanes: 1, CTA starting material; 2, CTB starting material; 3, reassembled holotoxin; 4, reassembled holotoxin nicked with trypsin.
