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Effects of specific amino acid substitutions in CTA and CTB on assembly of CT holotoxin in vitro. (A) Assembly of wild-type CTA (1 to 3) or CTA-F223D (4 to 6) with wild-type CTB. d-galactose resin flowthrough (1 and 4); eluate 1 (2 and 5), and eluate 2 (3 and 6). (B) Assembly of wild-type CTA with wild-type (1 to 3), I74D (4 to 6), or T78D (7 to 9) CTB. d-Galactose resin flowthrough (1, 4, and 7), eluate 1 (2, 5, and 8), and eluate 2 (3, 6, and 9).
