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. 2003 Jul;71(7):3831–3836. doi: 10.1128/IAI.71.7.3831-3836.2003

FIG. 2.

FIG. 2.

Western blot of auxotrophic S. enterica serovar Typhimurium cell lysates and culture supernatants separated by SDS-PAGE and probed with anti-PA monoclonal antibody. ZpVDL9.3PA83ec (plasmid-borne PA gene), ZpUTKmPA83ec (chromosomal PA gene), and Zoosaloral H (parent) were cultured overnight in Luria broth with appropriate antibiotic selection. Harvested cells and supernatants were normalized to similar CFU per milliliter and lysed in SDS-PAGE sample buffer. Lane M, Bio-Rad biotinylated SDS-PAGE standards, low range; lane 1, 1 mg of recombinant PA/ml; lane 2, Zoosaloral H cell lysate; lane 3, ZpVDL9.3PA83ec cell lysate; lane 4, ZpUTKmPA83ec cell lysate; lane 5, Zoosaloral H supernatant; lane 6, ZpVDL9.3PA83ec supernatant; lane 7, ZpUTKmPA83ec supernatant.