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. 2003 Jul;71(7):3837–3843. doi: 10.1128/IAI.71.7.3837-3843.2003

FIG. 2.

FIG. 2.

Western blot analysis of recombinant HpaA and Omp18 expressed in HK293 cells. (A) The purified recombinant antigens and lysate from untransfected HK293 cells were separated on a 4 to 12% Tricine-SDS-polyacrylamide gel and detected either by Coomassie blue staining (left lanes) or with a murine antibody against the His tag (αHis, 1:5,000) (right lanes). The secondary antibody was peroxidase-labeled anti-mouse IgG (1:20,000). The immunoblots were developed with a chemiluminescence system. The molecular masses are indicated on the right. (B) The purified recombinant antigens were separated on 10% tricine-SDS-polyacrylamide gels, transferred to nitrocellulose, and probed with five H. pylori-positive sera (P1 to P5, 1:500) or five sera from H. pylori-negative individuals (N1 to N5, 1:500). As controls, both recombinant proteins were detected with a murine antibody against the His tag (αHis, 1:5,000). The secondary antibody was peroxidase-labeled anti-human/anti-mouse IgG (1:20,000). The immunoblots were developed with a chemiluminescence system. The molecular masses are indicated on the right.