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Indirect targeted scission of the lac UV5 open complex (labeled OC). Lanes 1–8 contain 15 μM OP-5′-UGGAA-3′ in addition to the competing ORN. Lane 1: −9:+2; lane 2: −6:+2; lane 3: −5:+2; lane 4: −4:+2; lane 5: −3:+2; lane 6: trp EDCBA (−3:+2); lane 7: lac UV5 (−4:+1) top; lane 8: OP-5′-UGGAA-3′ only; lane 9: G + A ladder; lane 10: DNase I no RNAP; lane 11: DNase I + RNAP; lane 12: lac UV5 only.
