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(A) OP linked to either the 5′, 2′, or 3′ position of 5′-GUGGA-3′. 1) OP-5′-GUGGA-3′. 2) 5′-GU(OP)GGA-3′. 3) 5′-GUGGA-3′-OP. (B) Direct targeted scission of the lac UV5 open complex by 5′, 2′, and 3′ OP derivatives of 5′-GUGGA-3′. The open complex has been designated OC. Lane 1: lac UV5 scission by 30 μM 5′-GUGGA-3′-OP; lane 2: lac UV5 scission by 30 μM 5′-GU(OP)GGA-3′; lane 3: lac UV5 scission by 15 μM OP-5′-GUGGA-3′; lane 4: G + A ladder; lane 5: lac UV5 only.
