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Direct targeted scission of an artificial open complex with (5φOP)2Cu+ and OP-5′-UGGAA-3′. The open complex has been designated OC. Lane 1: Scission by 30 μM OP-5′-UGGAA-3′, no RNAP; lane 2: scission by 30 μM OP-5′-UGGAA-3′ + RNAP; lane 3: scission by 50 μM (5φOP)2Cu+ no RNAP; lane 4: scission by 50 μM (5φOP)2Cu+ + RNAP; lane 5: DNase I, no RNAP; lane 6: DNase I + RNAP; lane 7: labeled artificial bubble only; lane 8: G + A ladder of artificial bubble template strand.
