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BRCA1 binds core Pol II in vitro. Purified calf thymus Pol II was incubated with PinPoint protein (pp; lane 2) or PinPoint-BRCA1 fusion proteins (lanes 3 and 4) immobilized on streptavidin beads. After washing in buffer containing 750 mM KOAc and 0.5% Nonidet P-40, bound proteins were resolved by SDS/PAGE and were visualized by immunoblot analysis using a Pol II RPB1-specific antibody. Lane 1 represents 10% of total Pol II in binding reactions.
