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Pol II subunits hRPB10α and hRPB2 mediate VP16-dependent transcriptional activation. All reactions were performed as in Fig. 2 except that GAL4-VP16 was used as an activator. (A) Effect on VP16-dependent activated transcription by hRPB3 to hRPB11. For comparison, the activation in lane 2 (absence of added Pol II subunit) is 12-fold. (B) Effect on VP16-dependent activated transcription by hRPB2 peptides.
